Calcium Spiking in Plant Root Hairs Responding to Rhizobium Nodulation Signals
نویسندگان
چکیده
منابع مشابه
Calcium Spiking in Plant Root Hairs Responding to Rhizobium Nodulation Signals
SUMMARY Rhizobium lipochitooligosaccharide signal molecules stimulate multiple responses in legume host plants, including changes in host gene expression, cell growth, and mitoses leading to root nodule development. The basis for signal transduction in the plant is not known. We examined cytoplasmic free calcium in host root hairs using calcium-sensitive reporter dyes. Image analysis of injecte...
متن کاملIon changes in legume root hairs responding to Nod factors.
Departamento de Biologı́a Molecular de Plantas, Instituto de Biotecnologı́a, Universidad Nacional Autónoma de México, Apartado Postal 510–3, Cuernavaca Morelos 62271, México (L.C., F.S., C.Q.); Biology Department, Morrill Science Center, University of Massachusetts, Box 35810, Amherst, Massachusetts 01003–5810 (T.L.H.-C., J.G.K., P.K.H.); Instituto Gulbenkian de Ciência, P–2780–156 Oeiras, Portug...
متن کاملMicrotubule dynamics in living root hairs: transient slowing by lipochitin oligosaccharide nodulation signals.
The incorporation of a fusion of green fluorescent protein and tubulin-alpha 6 from Arabidopsis thaliana in root hairs of Lotus japonicus has allowed us to visualize and quantify the dynamic parameters of the cortical microtubules in living root hairs. Analysis of individual microtubule turnover in real time showed that only plus polymer ends contributed to overall microtubule dynamicity, exhib...
متن کاملA nuclear-targeted cameleon demonstrates intranuclear Ca2+ spiking in Medicago truncatula root hairs in response to rhizobial nodulation factors.
Lipochitooligosaccharide nodulation factors (NFs) secreted by endosymbiotic nitrogen-fixing rhizobia trigger Ca(2+) spiking in the cytoplasmic perinuclear region of host legume root hairs. To determine whether NFs also elicit Ca(2+) responses within the plant cell nucleus we have made use of a nucleoplasmin-tagged cameleon (NupYC2.1). Confocal microscopy using this nuclear-specific calcium repo...
متن کاملA Nuclear-Targeted Cameleon Demonstrates Intranuclear Ca Spiking in Medicago truncatula Root Hairs in Response to Rhizobial Nodulation Factors
Lipochitooligosaccharide nodulation factors (NFs) secreted by endosymbiotic nitrogen-fixing rhizobia trigger Ca spiking in the cytoplasmic perinuclear region of host legume root hairs. To determine whether NFs also elicit Ca responses within the plant cell nucleus we have made use of a nucleoplasmin-tagged cameleon (NupYC2.1). Confocal microscopy using this nuclear-specific calcium reporter has...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cell
سال: 1996
ISSN: 0092-8674
DOI: 10.1016/s0092-8674(00)81234-9